Relative quantification of ToFZY mRNA in different tomato tissues. Ct and amplification efficiency values were processed with the qBase software. Normalization factors were calculated as the geometric mean of the expression levels of three control genes (CAC, TIP41 and Expressed). The sample that showed the highest expression level was used as calibrator. cDNA samples came from the same set used in the evaluation of normalization: DMR, distal mature root; L1, younger leaf; L6, older leaf; I1, 1 mm bud; I9, open flower. Error bars show the standard deviation of three technical replicas.