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Table 4 Differentially expressed proteins in apoplastic washing fluids of leaves of oilseed rape (Brassica napus var. napus) after infection with Verticillium longisporum VL43.

From: Defence reactions in the apoplastic proteome of oilseed rape (Brassica napus var. napus) attenuate Verticillium longisporumgrowth but not disease symptoms

Spot no

Similarity (Organism) Peptide

NCBI (ACC)

Protein score (threshold)

MW

Target peptide

Factor

P

21

basic glucanase (Bj)

LYDPNQEVLSALR

YISVGNEVQPSDPTSR

GISGFPPSSGTFTPEFR

gi|86371164

(ABC94638)

223 (56)

30722

S (RC 1)

3.9

0.037

 

β-1,3-glucanase (Ath)

LASSQAEADTWVR

GISGFPPSSGTFTPEF

gi|6735303

(At3g57240)

91 (56)

37632

S (RC 1)

  

22

basic glucanase (Bj)

IASSQAEADTWVR

LYDPNQEVLSALR

YISVGNEVQPSDPTSR

GISGFPPSSGTFTPEFR

NLFHALVDTVYAALEK

GSNIDLLLDVPNPDLQR

LDYALFTSPSTVVNDGSNAYR

gi|86371164

(ABC94638)

405 (56)

30722

S (RC 1)

3.1

0.001

 

β-1,3-glucanase (Ath)

LASSQAEADTWVR

GISGFPPSSGTFTPEFR

TYVNNLIQTVK

gi|6735303

(At3g57240)

180 (56)

37632

S (RC 1)

  

23

β-1,3-glucanase (Ath)

LASSQAEADTWVR

GISGFPPSSGTFTPEFR

TYVNNLIQTVK

GSNIELVLDVPNPDLQR

gi|6735303

(At3g57240)

222 (56)

37632

S (RC 1)

4.8

0.049

30

not identified

    

5.8

0.007

32

not identified

    

8.3

0.000

34

basic endochitinase (CHB4) (Bn)

DSFINAANTFPNFANSVTR

AINGMECNGGNSGAVNAR

gi|584929

(Q06209)

95 (56)

29684

S (RC 1)

7.7

0.009

36

not identified

    

4.8

0.014

51

basic endochitinase (CHB4) (Bn)

DSFINAANTFPNFANSVTR

AINGMECNGGNSGAVNAR

DYCGQLGVDPGPNLS

gi|584929

(Q06209)

159 (44)

29684

S (RC 1)

4.2

0.020

52

not identified

    

3.5

0.045

53

not identified

    

4.4

0.005

54

Korean horseradish peroxidase (Rs)

MGDISPLTGSSGEIR

gi|1518388

(X91172)

94 (44)

33993

S (RC 3)

2.4

0.040

 

Peroxidase (Ath)

MGDISPLTGSSGEIR

gi|15239075

(At5g05340)

94 (44)

34650

S (RC 1)

  

55

not identified

    

3.6

0.005

  1. Data indicate means of 5 gels per treatment. Spot numbers refer to spots in Figure 7. NCBI and accession number (ACC) of the proteins were identified by comparing the peptide sequence with NCBI databank. Protein score, predicted molecular weight (MW) and predicted target peptide with reliability class (RC) are indicated. Factor indicates mean intensity of spots in apoplastic washing fluid of VL43-infected plants/mean intensity of spot in apoplastic washing fluid of control plants. P-values were calculated using ProteinWeaver software. Ath = Arabidopsis thaliana, Bj = Brassica juncea, Bn = Brassica napus, Rs = Raphanus sativus,