Figure 3

Example of allelic ladder developed for the marker VChr13a. The first electropherogram reports the ladder produced by mixing amplicons from the individual amplification of DNA from four cultivars, whose individual electropherograms are reported below and whose alleles cumulatively represent all alleles found in the population of this study. The sizes of the alleles are expressed in bp. Electrophoresis and analysis were performed on a MegaBace 500 Sequencer analyzer (GE HealthCare) using the ET-400-R size standard.