Figure 2

Electrophoretic pattern of true alleles (peaks with blue dots on top) and stuttering peaks of a tetra-nucleotide microsatellite (VChr5c) identified in this work and a di-nucleotide microsatellite (VVS2) currently used for grapevine fingerprinting[15]. Panel A and B report for each marker the composite pattern of all alleles found in the population of 48 cultivars of this study, obtained by graphical overlap of individual patterns of panel C. Blue arrow points to the homozygous allele in the sequenced genome of PN40024 for VChr5c, whose number of core repeats (8) is used for coding all other alleles accordingly, as described in the text. The allele coded N.D. is likely generated by a deletion in the sequence intervening the primer sites. Panel C reports the individual electropherograms of 4 and 8 cultivars which cumulatively represent all of the alleles for VChr5c and VVS2, respectively, found in a set of 48 cultivars.