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Table 4 Ion values (mM) for four treatments, including MS medium, used to solve the linear programming algorithm utilized by ARS-Media.

From: The effects of nitrogen and potassium nutrition on the growth of nonembryogenic and embryogenic tissue of sweet orange (Citrus sinensis(L.) Osbeck)

Ion

MS

1

2

3

B(OH)3

0.100259

0.100259

0.100259

0.100259

Ca(2+)

2.992884

2.992884

2.992884

2.992884

Cl(-)

5.985982

5.985982

5.985982

5.985982

Co(2+)

0.000105

0.000105

0.000105

0.000105

Cu(2+)

0.0001

0.0001

0.0001

0.0001

EDTA

0.100027

0.100027

0.100027

0.100027

Fe(2+)

0.099997

0.099997

0.099997

0.099997

I(-)

0.004999

0.004999

0.004999

0.004999

K(+)

20.04693

7.5

25

15

Mg(2+)

1.501201

1.501201

1.501201

1.501201

Mn(2+)

0.099989

0.099989

0.099989

0.099989

Mo(2-)

0.001033

0.001033

0.001033

0.001033

NH 4 (+)

20.61395

2.5

25

15

NO 3 (-)

39.40666

10

50

30

Na(+)

3.202475

3.202475

3.202475

3.202475

PO4(3-)

1.249219

1.249219

1.249219

1.249219

SO4(2-)

1.738084

1.738084

1.738084

1.738084

Zn(2+)

0.036797

0.036797

0.036797

0.036797

  1. The results are the salt/acid/base formulations required to make each treatment solution. All ions other than the three being varied (italicized and bold) are fixed and unvaried, which illustrates an experimental design free of ion confounding.