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Figure 1 | BMC Plant Biology

Figure 1

From: Identification of precursor transcripts for 6 novel miRNAs expands the diversity on the genomic organisation and expression of miRNA genes in rice

Figure 1

Expression profile of novel miRNA candidates and dependence on OsDCL1. A) Northern blot analysis was carried on using total RNAs from the indicated tissues and complementary probes to the candidate miRNAs (see methods). A control for expression of conserved osa-miR159a was also included. S, seedlings; L, mature leaves; Im. pn, immature panicles; Fec. Pn: panicles after fertilisation; R, roots. U6 is a control for RNA loading made with a probe complementary to U6 snRNA from Arabidopsis which is conserved in all plants. B) Northern blot analysis for different miRNA candidates was carried on total RNA extracted from rice seedling from wild type and osdcl1IR lines as indicated. Dependence of osa-miR1874, expressed only in panicles, could not be tested in the osdcl1IR lines as these have low fertility and produce small panicles.

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