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Figure 4 | BMC Plant Biology

Figure 4

From: The MYST histone acetyltransferases are essential for gametophyte development in Arabidopsis

Figure 4

Analysis of gametophyte development in ham sesquimutants. (A-F) Analysis of pollen development by DAPI staining. Pollen grains were released from anthers, stained with DAPI, and visualized under a light microscope equipped with a UV fluorescent filter. At uni-nucleate stage, microspores of wild-type (A) and HAM1/ham1-1; ham2/ham2 plants (D) were indistinguishable. In contrast to wild-type microspores (B and C), HAM1/ham1-1; ham2/ham2 plants contain approximately one-half of arrested microspores (arrows) at the bicellular (E) and tricellular pollen stages (F). Bars: 10 μm. (G-J) Cleared whole mounts of wild-type and ham sesquimutant ovules. Mature ovules at stage G7 of wild-type (G) and HAM1/ham1-1; ham2/ham2 mutant (H). Bars: 40 μm. Images I and J are enlargements of boxed sections in G and H respectively. Bars: 20 μm. (I) Magnified section shows the presence of central cell nucleus, two synergid nuclei and the egg cell nucleus in wild-type ovule. (J) An ovule from ham sesquimutant at the same stage showing the presence of only one nucleus (white arrow). No other nuclei were visualized at other focal planes.

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