Figure 5

Dirunal changes in At5g39790 protein in spinach leaf. Spinach plants were grow in 12 hour cycle of light and dark and harvested at the times shown. Samples were processed, run on 12% SDS-PAGE, blotted and probed with antibodies generated against At5g39790. The open and solid bars below the blot indicate if samples were taken in the light (open bar) or dark (solid bar). The equivalent of 7.5 mg fresh weight leaf extract was analyzed for each sample.