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Figure 4 | BMC Plant Biology

Figure 4

From: Arabidopsis At5g39790 encodes a chloroplast-localized, carbohydrate-binding, coiled-coil domain-containing putative scaffold protein

Figure 4

Effect of pH, magnesium and redox state on starch binding of At5g39790. Purified At5g39790 was incubated with starch as a function of pH in PBS as described in Methods. Samples were processed as in Fig. legend 3. Only the pellets are illustrated: (a) incubation of At5g39790 as a function of pH in phosphate buffered saline (PBS); (b) incubation of At5g39790 as a function of Mg2+ concentration in PBS, pH 7.3) (the 0 mM Mg2+ point is the same as in Fig 4a, lane 3); (c) incubation of At5g39790 under day (reducing [100 mM DTT], pH 8.0, 1 mM Mg2+) and night (oxidizing [30 mM H2O2], pH 7.0, 1 mM Mg2+) stromal conditions.

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