Skip to main content

Table 6 Cloning primers and PCR conditions.

From: Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity

Gene

Primersa

Pfub Tm/cycles

Taq Tm/cycles

Reference sequence

Mal d 1.01

F: ATCTCCAACACAATACTCTCAAC

R: AAAGCCACACAACCTTCGAC

58/25

60/2

AY789236

Mal d 1.02

F: CATCCTTGGTAGTTGCTTTC

R: ACCATAGAAACATATTAATTTAGT

52/25

54/2

AY789239

Mal d 1.04

F: CGTAGTTGGACAAGTGTCTTAGT

R: AGGGTAACACACAAATTACATG

58/30

60/2

AY789242

Mal d 1.05

F: AGTTCATCATGGGTGTTTTC

R: GGTAACACACAAATTACAAATATGC

53/30

55/2

AY789245

Mal d 1.06A-C

F: CATGGGTGTCCTCACATACGAAAC

R: TTAGTTGTAGGCATCAGGATTG

55/25

57/2

AY789248

Mal d 1.06C

F: ATGGGTGTCCTCACATACGAAACT

R: TTAGTTGTAGGCATCAGGATTGGCCACAAGGTG

62/30

64/2

AY789255

  1. aPrimers for Mal d 1.04 and Mal d 1.05 are new, others have been adopted from Gao et al. [15].
  2. b The PCR was performed in two steps starting with Pfu polymerase and finishing with super Taq [15, 22].