Assessment of allelic diversity in intron-containing Mal d 1 genes and their association to apple allergenicity

BMC Plant Biology

Table 1 Genetic variation in the intron containing Mal d 1 genes of linkage groups 6 (Mal d 1.05) and 13 (Mal d 1.01) among ten apple cultivars.

Iso-allergen	Variant^a	Sil. mut	gDNA #^b	Cultivar^c										GenBank Accessions
				GD	PS	IM	CO	JO	RD	FJ	DS	PM	FS
Mal d 1.05	01	1	1	+^d	+	+ +	+ +	+	+		+	+ +	+	AY789245–AY789246, AY827676–AY827682
	02	1	2								+		+	AY789247, AY827683
	02	2	3	+	+									AY827684–AY827685
	03	1	4					+	+	+ +				AY827686–AY827688
Mal d 1.01	05	1	1									+		AY789236
	05	1	2	+				+		+				AY827639–AY827641
	05	2	3	+	+	+ +	+ +	+			+ +		+ +	AY789238, AY827633–AY827638
	05	3	4		+							+		AY789237, AY827642
	05	4	5						+	+				AY827643–AY827644
	09	1	6						+					AY827645

^aVariants refer to different protein sequences. Variant numbers of Mal d 1.01, Mal d 1.02 and Mal d 1.04 are according to the allergen list (http://www.allergen.org/Allergen.aspx; Sept 2008), those for Mal d 1.05 and Mal d 1.06 are according to Gao et al. [15].
^bGenome sequences are numbered successively per gene
^cGolden Delicious (GD), Priscilla (PS), Ingrid Marie (IM), Cox (CO), Jonathan (JO), Red Delicious (RD), Fuji (FJ), Discovery (DS), Prima(PM) and Fiesta (FS). Note that different accessions of Priscilla seem to exist. The Priscilla used here is a parent of Santana as confirmed by 20 SSR markers (unpublished data), but can not descend from its supposed mother Starking Delicious http://www.hort.purdue.edu/newcrop/pri/coop04.html
^d Indicates the presence of an allele in heterozygous (+) or homozygous (+ +) condition.

ISSN: 1471-2229