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Table 2 Brachypodium reference genes for normalization and their expression stability values calculated by the NormFinder software

From: Exploring valid reference genes for gene expression studies in Brachypodium distachyonby real-time PCR

Rank

Tissue

Growth hormone

Heat/Cold

High salt/Drought

Developmental stage

Total

 

Gene

Stability

Gene

Stability

Gene

Stability

Gene

Stability

Gene

Stability

Gene

Stability

1

Ubi4

0.0846

Ubi10

0.1377

GAPDH

0.1723

SamDC

0.0372

Ubi4

0.0987

UBC18

0.2586

2

Ubi10

0.0846

Ubi4

0.1640

EF1α

0.1951

UBC18

0.0378

SamDC

0.0996

Ubi4

0.3363

3

GAPDH

0.2751

GAPDH

0.1768

SamDC

0.2164

GAPDH

0.0738

UBC18

0.156

EF1α

0.3876

4

EF1α

0.2796

ACT7

0.2053

ACT7

0.2434

EF1α

0.1951

GAPDH

0.2276

SamDC

0.4791

5

ACT7

0.3708

TUA6

0.2103

RCA

0.2689

RCA

0.2063

ACT7

0.3401

ACT7

0.4845

6

UBC18

0.5032

UBC18

0.2291

Ubi10

0.3139

Ubi10

0.3426

RCA

0.3682

TUA6

0.5777

7

SamDC

0.5512

EF1α

0.3028

TUA6

0.3279

ACT7

0.3441

Ubi10

0.3776

Ubi10

0.5937

8

RCA

0.8238

SamDC

0.3946

UBC18

0.3309

Ubi4

0.4078

EF1α

0.6029

GAPDH

0.6658

9

TUA6

1.3712

RCA

0.7124

Ubi4

0.8483

TUA6

0.7401

TUA6

0.6579

RCA

0.926

  1. In each column, a low stability value as an estimate of the combined intra- and intergroup variation of the respective gene represents a high gene expression stability of the respective gene between the matched samples.