Molecular population genetics and gene expression analysis of duplicated CBF genes of Arabidopsis thaliana

BMC Plant Biology

Table 3 Evolutionary distances between paralogs and orthologs in non-coding and coding regions

Region	K	K_S	K_a	K_a/K_S
Promoters ^a (paralogs)		N.A	N.A	N.A
CBF1 vs. CBF2	0.448 ± 0.043
CBF2 vs. CBF3	0.360 ± 0.035
CBF1 vs. CBF3	0.501 ± 0.045
UTR^b (paralogs)
CBF1 vs. CBF2	0.212 ± 0.041
CBF2 vs. CBF3	0.156 ± 0.033
CBF1 vs. CBF3	0.266 ± 0.047
CDS (paralogs)	N.A.
CBF1 vs. CBF2		0.408 ± 0.069	0.062 ± 0.012	0.152
CBF2 vs. CBF3		0.462 ± 0.078	0.068 ± 0.014	0.147
CBF1 vs. CBF3		0.546 ± 0.093	0.064 ± 0.013	0.117
CBF1 vs. CBF4		1.478 ± 0.451	0.216 ± 0.031	0.146
CBF2 vs. CBF4		1.728 ± 0.654	0.233 ± 0.032	0.135
CBF3 vs. CBF4		1.456 ± 0.431	0.232 ± 0.030	0.159
CDS (orthologs^c)
AtCBF2 vs. AlCBF2		0.340 ± 0.059	0.048 ± 0.012	0.141
AtCBF3 vs. AlCBF3		0.284 ± 0.056	0.045 ± 0.011	0.158

^a Regions containing 500 bp upstream from the transcriptional unit (TU) were analyzed; K, average sequence distance between promoter regions of CBFs; ^b the 5' and 3' untranslated regions (UTRs) were analyzed; K_s, average distance between CBFs estimated using synonymous sites; K_a, average distance between CBFs estimated using nonsynonymous sites; N.A., not allowed. ^c AlCBF2 (accession nos.: EF523193~EF523195) and AlCBF3 (accession no.: EF523196).

ISSN: 1471-2229