Genome-wide transcriptional analysis of super-embryogenic Medicago truncatulaexplant cultures

BMC Plant Biology

Table 1 Comparison of real-time RT-PCR and microarray results for selected genes

Probe ID	Annotation	Microarray (log2)	RT-PCR (log2)
Mtr.47631.1.S1_s_at	Transposase	1.43 ± 0.28	4.22 ± 0.53
Mtr.15107.1.S1_at	RNA-directed DNA polymerase	1.37 ± 0.14	4.39 ± 0.89
Mtr.45925.1.S1_s_at	GH	1.32 ± 0.43	6.92 ± 0.58
Mtr.43735.1.S1_at	MtRR1	1.10 ± 0.30	2.77 ± 0.17
Mtr.47174.1.S1_at	AGL20	1.06 ± 0.10	4.84 ± 0.16
Mtr.41073.1.S1_at	FPF1	1.32 ± 0.14	3.33 ± 0.37
Mtr.8427.1.S1_at	LipOx	1.19 ± 0.34	3.10 ± 0.53
Mtr.10439.1.S1_at	EIN3	-1.41 ± 0.11	-2.76 ± 0.09
Mtr.8585.1.S1_at	MtN3	1.71 ± 0.08	6.24 ± 0.28
Mtr.18380.1.S1_at	Fasciclin	1.52 ± 0.02	3.9 ± 0.03

These M. truncatula probe set IDs are from the Medicago GeneChip. GH, Glycoside hydrolase (note this gene also incorrectly annotated as Regulator of chromosome condensation on the array); RR, response regulator; AGL20, agamous-like 20; FPF, flower promoting factor; LipOx, lipoxygenase; EIN, ethylene-insensitive; MtN3, M. truncatula nodulin3. Values shown are ratios of the means of three independent measurements from microarray data or real-time RT-PCR data. Note the Log₂ changes are given rather than fold changes. Standard deviations are given as ± values.

ISSN: 1471-2229