Figure 5

M. sexta feeding significantly reduces Pst DC 3000 disease spread. Values are mean (± SE) colony-forming units (CFUs) of Pst DC3000 after inoculation of the leaves of WT/irdefensin (76 and 96) plants that were either uninduced or previously attacked by M. sexta larvae 4 days earlier. To record the CFUs, surface-sterilized leaf discs (1 cm²) were ground in 1 ml sterile water and 40 μl of supernatant was spread on plates containing LB agar + antibiotics (rifamycin and tetracycline). Colonies were counted after 48 h of incubation at 28°C. Different letters in lower and upper cases indicate significant differences among Pst DC3000-inoculated WT plants and the transgenic plants on days 2 and day 4, respectively (N = 5).