Figure 4

The number of Pst DC3000 colonies quantified in the guts of M. sexta larvae that fed on the Pst DC3000-inoculated WT and ir defensin (76 and 96) plants do not differ. A) Mean (± SE) colony-forming units (CFUs) of Pst DC3000 in the guts of larvae that fed on either the Pst DC3000-inoculated WT/irdefensin (76 and 96) or uninduced WT/irdefensin (76 and 96) plants. The larval guts from 5 replicate larvae that fed on either Pst DC3000-inoculated WT and irdefensin (76 and 96) for 12 days were surgically removed and ground in 1 ml sterile water. 40 μl of supernatant was spread on plates containing LB agar plate containing rifamycin and tetracycline to select for the growth of Pst DC3000. Colonies were counted after 48 h of incubation at 28°C. B) Photographs of LB plates + antibiotics (rifamycin and tetracycline) showing Pst DC3000, in addition to four unidentified/unknown microorganisms that could also grow on LB plants supplemented with antibiotics (N = 5).