Figure 2

T-DNA insertional mutants of RACK1B and RACK1C. (A) A diagram to illustrate the T-DNA insertion sites in rack1b-1 and rack1b-2 mutants. (B) RT-PCR analysis of RACK1B transcript in rack1b mutants. RACK1B-specific primers that amplify the full-length transcript of RACK1B in wild-type (Col) were used. (C) The rosette morphology of rack1b and rack1c mutants. Shown are plants grown 48 days under 10/14 h photoperiod. (D) A diagram to illustrate the T-DNA insertion sites in rack1c-1 and rack1c-2 mutants. (E) RT-PCR analysis of RACK1C transcript in rack1c mutants.RACK1C-specific primers that amplify the full-length transcript of RACK1C in Col were used. Gray boxes in (A) and (D) represent coding regions and white boxes represent 5'-UTR and 3'-UTR regions. The T-DNA inserts are not drawn to scale. LB, T-DNA left border. Total RNA isolated from 10 d-old, light-grown seedlings was used for RT-PCR analysis in (B) and (E). RT-PCR was performed with 30 cycles. The expression of ACTIN2 was used as a control.