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Table 2 Physiological features of C. reinhardtii apr mutant strains and the respective wild types.

From: A novel screening protocol for the isolation of hydrogen producing Chlamydomonas reinhardtiistrains

 

WT (CC-425)

WT (CC-124)

apr1

apr4

apr6

apr7

P/R ratio at saturated light intensities

6.7 ± 1.7

4.2 ± 0.41

1.5 ± 0.1

0.6 ± 0.4

0.4 ± 0.1

3.5 ± 0.7

respiration rate (nmol O2 × h-1 × μg Chl-1)

17.7 ± 3.2

35.6 ± 0.5

40.5 ± 4.9

14.7 ± 1.9

22.2 ± 2.7

22.7 ± 7.3

maximal O2 production rate (nmol O2 × h-1 × μg Chl-1)

97.1 ± 13.8

114.8 ± 16.0

20.6 ± 5.2

-6.1 ± 5.9

-6.8 ± 0.7

54.6 ± 13.8

compensation point (μE × m-2 × s-1)

27.2 ± 6.4

39.1 ± 6.8

53.1 ± 2.3

-

-

36.3 ± 5.1

light sensitive

no

no

no

yes

no

High light sensitive

Ability of photoautotrophic growth (TBP medium)

yes

yes

yes

no

no

yes

Fv/Fm

0.66 ± 0.01

0.66 ± 0.023

0.58 ± 0.02

0.54 ± 0.01

0.00 ± 0

0.30 ± 0.01

  1. The compensation point reflects the light intensity at which the cultures produce as much oxygen as they consume. Exposing sealed cultures to lower light intensities than at the compensation point lead to anaerobiosis. Values represent means ± standard deviation (n = 3).