Characterization of sis7 molecular lesions. (A) Mutations in SIS7/NCED3/STO1. The molecular lesions associated with the three sis7/nced3/sto1 mutants identified as part of this work are shown. Nucleotides are numbered with respect to the start codon. (B) SIS7 transcript levels in wild-type and sis7 plants. RT-PCR was used to amplify SIS7/NCED3/STO1 transcripts from 4-week old wild-type (Col) and sis7 mutant plants. 2 μL cDNA was used as a template for the first PCR reaction (35 cycles). ACTIN transcripts were amplified as a positive control.