A: Multiple alignment of the deduced Ole e 1 amino acid sequences displayed in Figs 1, Additional file 1, and the amino acid sequence of Ole e 1 experimentally determined by Edman degradation (20). Conserved Cys at positions 22, 43, 78, 90 and 131 are pointed by arrows (discontinuous line arrow for the semi-conserved 19C). Predicted N-glycosylation motifs are indicated by boxes. Immunodominant T-cell epitopes (A, B and C), experimentally determined by  are showed by brackets. IgE and IgG B-cell immunodominant regions of Ole e 1, determined by  are respectively shown by stars and bars. Numbering follows the experimental Ole e 1 sequence obtained by Edman degradation. B: Sequence conservation index calculated by Jalview programme according to . Conservation is measured as a numerical index (0 to 10) reflecting the conservation of physico-chemical properties for each column of the alignment: identities score highest, and the next most conserved group contain substitutions to amino acid lying in the same physico-chemical class.