Table 4 PCR primer pairs used to amplify microsatellites in wild species of Arachis
Locus | Motifs | Primer sequences (5'-3') | Expected size (bp) | Annealing temperature (°C) | MgCl2mM | |
|---|---|---|---|---|---|---|
| Â | Â | Forward | Reverse | Â | Â | Â |
Ah2 | (AC)10 | TTACACGGTAGCCCATTTCC | CCAAACCACAATTCAGTAGCAA | 199 | 55 | 2.5 |
Ah3 | (GA)15.(AG)7.(GT)7.(GA)7 | TCGGAGAACAAGCACACATC | TTGCGCTCTTTCTCACACTC | 202 | 55 | 1.5 |
Ah7 | (TG)8 | CAGAGTCTGTGATTTGTGCACTG | ACAGAGTCGGCCGTCAAGTA | 102 | 55 | 1.5 |
Ah11 | (TTA)15 | AAATAATGGCATACTTGTGAACAATC | TTCCACCAAGGCAAGACTATG | 176 | 55 | 2.5 |
Ah19 | (TA)18 | CCCTCGAAGGTGGAATTCAT | CGGGGATTGTTCGAGTTTG | 197 | 55 | 2.5 |
Ah20 | (TG)10 | TGCATGTCTCTTGTAACTTAATACACT | TTCATGTCAATGATGTTTCCAA | 197 | 55 | 2.5 |
Ah21 | (GAA)9 | CTTGGAGTGGAGGGATGAAA | CTCACTCACTCGCACCTAACC | 109 | 55 | 1.5 |
Ah23 | (AT)19 | GAAGGTGGAATTCATTCTCAAAA | TTCGAGTTTGAACAACTGACG | 183 | 55 | 2.0 |
Ah26 | (CT)14 | GAAAATGATGCCATAAAGCGTA | AGTGTAACACCCCGTTAGCC | 182 | 55 | 2.0 |
Ah30 | (GA)9 | TGCTCTTCTTTTCCTTTTCAC | AACGGCCAAAACTGAAATTA | 123 | 45 | 2.0 |
Ah51 | (AG)34 | CCTCTTCACAAGAGTGGACTATGA | CCCCCTCCTTTTGTTCTCTC | 154 | 55 | 2.0 |
Ah6–125* | (GAA)13 | TCGTGTTCCCGATTGTCC | GCTTTGAACATGAACATGCC | 180 | 55 | 2.0 |
Ah126 | (GA)8..(GA)9 | CCCTGCCACTCTCACTCACT | CGTACAAGTCAGGGGGTGAC | 187 | 60 | 1.5 |
Ah282 | (CCA)6..(AAG)6 | GCCAAACACACCACATTTCA | GGCTCCAATCCCAAACACTA | 203 | 55 | 2.5 |