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Table 2 Distribution of differentially expressed ESTs among the three tissue types. Non-redundant sequences (contigs and singleton ESTs) from each individual library were analyzed using the GO classification.

From: Comparative analysis of ESTs involved in grape responses to Xylella fastidiosa infection

Biological process

leaf

stem

shoot

 

lib-1

lib-4

lib-7

lib-8

lib-2

lib-5

lib-3

lib-6

lib-9

lib-11

lib-12

lib-10

 

Inf-Res

Cont-Res

Inf-Sus

Cont-Sus

Inf-Res

Cont-Res

Inf-Sus

Cont-Sus

Inf-Res

Cont-Res

Inf-Sus

Cont-Sus

Signal transduction and defense response

 

6.50

 

0.32

3.09

3.15

5.31

2.35

  

0.68

 

Response to stress & pathgenic fungi

  

0.85

 

0.34

0.39

 

0.52

 

0.97

0.34

 

Protein modification and targeting

1.92

 

2.54

 

1.37

1.97

 

0.78

2.56

0.97

0.68

 

Protein catabolism

         

0.32

0.34

 

Protein carbohydrate and fatty acid metabolism

0.96

0.81

 

0.63

1.03

0.39

 

1.04

 

0.65

0.68

 

Nucleic acid metabolism

 

0.81

  

0.34

0.79

 

0.52

 

0.32

0.68

 

Electron protein and other transport

0.96

0.81

0.85

1.27

0.69

3.54

0.88

3.13

 

1.62

1.69

2.30

Biosynthesis

  

1.69

0.32

 

0.39

 

0.52

 

0.32

1.02

 

Xenobiotic metabolism

 

3.25

  

0.34

0.39

13.27

0.26

 

0.32

 

1.15

Others

   

0.32

0.34

1.57

   

0.32

0.68

 

Cellular component

leaf

stem

shoot

 

lib-1

lib-4

lib-7

lib-8

lib-2

lib-5

lib-3

lib-6

lib-9

lib-11

lib-12

lib-10

Chloroplast

11.54

 

15.25

11.75

0.34

 

0.88

0.78

3.42

2.59

4.41

8.05

Integral to membrane

 

0.81

  

1.37

4.72

0.88

1.04

 

0.65

0.68

 

Nucleus

2.88

 

0.85

 

1.37

0.39

 

0.78

0.85

0.32

0.34

1.15

Membrane

1.92

   

0.69

1.57

 

0.52

  

1.69

 

Oxygen evolving complex

2.88

 

1.69

1.59

   

0.26

 

0.32

0.34

1.15

Nucleosome

2.88

 

1.69

0.32

0.69

  

0.52

    

Mitochondrion

0.96

   

0.69

0.39

 

0.52

 

0.32

1.02

 

Extracellular space

  

0.85

0.32

1.03

0.39

 

0.78

 

0.32

0.34

1.15

Golgi apparatus

    

0.34

0.39

 

0.26

 

0.65

0.68

 

Ubiquitin ligase complex

   

0.63

 

0.39

 

0.26

    

Phosphopyruvate hydratase complex

     

0.39

 

0.26

  

0.34

 

Protein phosphatase type 2A complex

     

0.39

 

0.00

 

0.65

  
 

leaf

stem

shoot

 

lib-1

lib-4

lib-7

lib-8

lib-2

lib-5

lib-3

lib-6

lib-9

lib-11

lib-12

lib-10

Cell wall

       

0.52

1.71

 

0.34

1.15

Microsome

       

0.52

  

0.34

 

Cytosol

    

0.34

  

0.26

   

1.15

Chromatin

  

0.85

       

0.34

 

Extracellular region

 

1.63

   

0.39

      

Mitochondrial inner membrane

0.96

         

0.34

 

Plasma membrane

   

0.32

    

0.85

   

Photosystem I

   

0.63

        

Photosystem I reaction center

  

0.85

0.63

        

Ribulose bisphosphate carboxylase complex

 

0.81

   

0.39

 

0.26

    

Golgi membrane

          

0.34

 

Cytoplasm

       

0.26

    

Mitochondrial outer membrane

          

0.34

 

Endoplasmic reticulum

       

0.26

    

Chloroplast inner membrane

          

0.34

 

Others

            

Molecular function

leaf

stem

shoot

 

lib-1

lib-4

lib-7

lib-8

lib-2

lib-5

lib-3

lib-6

lib-9

lib-11

lib-12

lib-10

Ubiquitin conjugating enzyme activity

    

1.03

       

Transporter activity

 

0.81

0.85

0.32

1.37

1.57

 

1.31

 

1.29

3.39

 

Transcription and translation factor activity

   

0.32

1.03

0.79

2.65

0.78

1.71

1.94

1.02

1.15

Structural constituent of ribosome

4.81

 

7.63

2.54

3.44

1.57

4.42

4.70

10.26

8.74

8.47

3.45

Siganl transducer and receptor activity

   

0.32

 

0.79

 

0.52

 

0.32

1.02

 

RNA binding

0.96

 

0.85

0.32

1.03

 

0.88

1.04

1.71

1.62

1.69

4.60

Nutrient reservoir activity

 

3.25

    

7.08

0.52

    

Molecular function unknown

1.92

0.81

1.69

 

1.03

0.79

0.88

0.52

 

0.65

1.36

2.30

Ligand binding/carrier

12.50

11.38

6.78

3.49

11.00

11.42

3.54

13.58

9.40

6.15

10.5

8.05

Enzyme inhibitor activity

 

0.81

 

0.32

0.34

0.39

 

1.04

 

0.65

  
 

leaf

stem

shoot

 

lib-1

lib-4

lib-7

lib-8

lib-1

lib-4

lib-7

lib-8

lib-1

lib-4

lib-7

lib-8

Catalytic activity

  

0.85

0.32

0.34

0.79

 

1.04

0.85

1.62

1.69

1.15

Others

0.96

 

0.85

0.63

0.69

1.97

2.65

0.78

2.56

1.62

1.36

 

Hydrolase activity, hydrolysing O-glycosyl compounds

 

2.44

0.85

0.32

2.06

1.57

0.88

1.04

2.56

2.27

2.03

1.15

Transferase activity

 

1.63

0.85

1.59

0.34

1.18

0.88

0.52

1.71

0.65

1.36

 

Protein kinase and phosphatase activity

2.78

 

0.85

 

1.03

1.18

 

0.52

1.71

0.97

3.39

1.15

Monooxygenase/oxidoreductase activity

10.58

1.63

10.17

3.17

0.69

1.18

3.54

2.87

6.84

2.59

2.71

2.30

GTP binding and GTPase activity

0.96

0.81

1.69

0.32

2.06

1.57

 

1.57

1.71

1.62

2.71

2.30

Isomerase activity

0.96

1.63

1.69

0.32

0.69

1.57

0.88

1.57

2.56

1.62

1.02

1.15

Endopeptidase activity

2.88

 

1.69

 

2.06

 

3.54

0.78

  

1.02

3.45

Dehydrogenase activity

   

0.32

0.69

0.39

 

0.52

0.85

 

0.34

 

Chitinase activity

 

1.63

 

0.32

0.69

1.57

 

0.52

 

0.32

0.68

 

Hits

68

52

77

107

133

134

60

203

63

143

191

43

No hits

36

71

41

208

157

120

53

180

54

166

104

44

Total sequences

104

123

118

315

290

254

113

383

117

309

295

87

% Unique(hits)

34.615

57.72

34.75

66.03

54.13

47.244

46.9

47

46.15

53.722

35.3

50.57