Figure 4

MSH7 RNAi transformation vector. Sense (630 bp) and antisense (880 bp) fragments of TaMSH7 create a hairpin loop RNA structure when transcribed. This dsRNA may then reduce HvMSH7 expression through RNAi. The construct contains a hygromycin resistance gene, hygromycin phosphotransferase (hpt), which was used as a selectable marker during tissue culture. This gene was also utilised for analysis of transgene segregation in the T₁ population.