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Figure 2 | BMC Plant Biology

Figure 2

From: TaMSH7: A cereal mismatch repair gene that affects fertility in transgenic barley (Hordeum vulgare L.)

Figure 2

Selected T 0 transgenic barley lines transformed with a MSH7 double-stranded RNAi construct. Lanes 1 to 7 – various Hvmsh7 transgenic lines (#26, 31, 41, 45, 46, 47, 49), lane 8 – transformed empty vector control, lane 9 – non-transformed barley control, lanes 10 to 15 – various Hvmsh7 transgenic lines (#50, 51, 52, 54, 55, 56), lane 16 – transformed empty vector control, lanes 17 and 18 – transgenic lines Hvmsh7–57 and 58 respectively. Copy numbers for selected lines represented on this blot (Hvmsh7-41, 50, 52, 55, 56, 57) and subsequently analysed by Q-PCR for MSH7 expression levels, are highlighted in Table 1.

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