MSH7 sequence alignments. (A) Three distinct sets of TaMSH7 sequences were identified which are representative of the three bread wheat genomes (A, B and D). The T. tauschii (Tt) sequence, CS D sequence and the N3B T3Ad sequence represent the D genome. The N3B T3Aa and CS A sequences represent the A genome, while the remaining sequence (CS B) represents the B genome. (B) The majority of differences in the sub-genomic amino acid sequence were at non-conserved residues. One change Leu → Ser at residue 596 of genome B (pink) was at a residue that is conserved amongst other MSH7 and MSH6 proteins and the prokaryotic homologue, MutS. (C) Two differences in amino acid sequence between the CS and ph2b D genome sequences were identified (pink). Both these amino acids were present in other MSH7 proteins.