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Table 5 Conditions and tissues used for mRNA extraction.

From: Sequencing analysis of 20,000 full-length cDNA clones from cassava reveals lineage specific expansions in gene families related to stress response

Treatment

Age

Tissue

Duration of treatment before RNA extraction

No treatment

9, 11, 12 weeks

leaf

 

No treatment

9 month

root

 

Drought shock

7-weeks

leaf

3, 6, 24, 72 hours

Heat

9-weeks

leaf

3, 6, 24, 72 hours

PPD

9 month

root

24, 48, 120 hours

High Al, low pH

9 weeks

leaf

3, 6, 24, 72 hours

High Al, low pH

9 month

root

6, 24, 48 hours