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Table 1 Acyl incorporation into PC and PE, comparing PAM with plasma membranes and an ER-rich fraction

From: LysoPC acyltransferase/PC transacylase activities in plant plasma membrane and plasma membrane-associated endoplasmic reticulum

 

Acyl incorporation from [14C]18:1-CoA (pmol·min-1·[mg protein]-1)

 

PC

PE

Plasma membrane

  

   on its own

53

23

   + lysoPC

549

47

   +lysoPC + AgNO3

7

6

PAM-decreased plasma membrane

  

   on its own

89

27

   + lysoPC

329

37

   + lysoPC + AgNO3

11

5

PAM

  

   on its own

74

20

   + lysoPC

840

49

   + lysoPC + AgNO3

14

10

Enriched ER

  

   on its own

91

13

   + lysoPC

899

53

   + lysoPC + AgNO3

15

8

  1. A pea shoot plasma membrane fraction was the starting material for the isolation of the PAM fraction. The PAM-decreased plasma membrane fraction represents the plasma membranes obtained after the removal of the PAM. The ER-enriched fraction is fraction number 1 from the 10-step counter current distribution (see Fig. 1). All fractions were incubated with [14C]18:1-CoA on its or in the presence of 70 μM lysoPC (acylated with 18:1), without or with 80 μM Ag NO3. Mean values are presented for 2–5 independent experiments, with standard deviations less than 10%.