Figure 7
The effects of phospholipase A 2 (PLA 2 ) and 18:1-CoA on the metabolism of exogenous PC in isolated plasma membranes. Plasma membranes, isolated from hypocotyls of dark-grown soybean, were incubated with 0.25 μM sn-1,2-[14C]18:1-PC (3.7 GBq/mmol) in buffer containing 0.05% (w/v) Triton X-100 for 30 min, without or with the indicated additions (0.001 U bee venom PLA2, 100 μM non-radiolabelled 18:1-CoA). A, The distribution of radiolabel between lysoPC (cross-hatched bar), PC (solid bar) and free fatty acids (diagonally hatched bar). B, The distribution of radiolabel between the sn-1 (diagonally hatched bar) and sn-2 (cross-hatched bar) positions of [14C]PC. The data represent the mean values and range from duplicates.