Figure 5
Effect of heterologous TgSATm expression on oxidative stress induced by different metals in A. thaliana shoot tissues from independent homozygous T4 lines transformed with vector containing TgSAT-m, S 4–9(black), S 3-1(grey) and S 5-4(white) or empty vector E 1–5(striped). Total shoot lipid peroxidation was measured as TBARS formed in response to each of the 4 different metal treatments after growth in normal media followed by transfer into 1/2 strength MS medium plates also containing 125 μM Ni (NO3)2, 125 μM Co (NO3)2, 125 μM Zn (NO3)2, and 75 μM Cd (NO3)2 for a period of 4 days. Values represent difference in TBARS compared to growth on control media without metals. Data represents averages (n = 6) ± SD (*p < 0.05; ** p < 0.01; *** p < 0.001, t-test).