Genomic organization of the guar conglutin gene. (A) Schematic diagram of the guar conglutin cDNA. (B) DNA gel blot analysis of guar conglutin. Genomic DNA was digested with SacI, SacI/EcoRI and HindIII restriction endonucleases. The first and last lanes represent 1 kb ladder molecular weight markers, the second through fourth lanes show guar genomic DNA digested with SacI, SacI/EcoRI and HindIII, respectively; the fifth through seventh lanes show the blot hybridized with the conglutin probe.(C). PCR analysis of the guar conglutin gene from cDNA and genomic DNA templates.