Table 1 Compounds used as agonists and antagonists to elucidate UV-B signal transduction pathway in Catharanthus roseus cultured cells
Chemical | Working concentration (stock solution) | Effect/References |
|---|---|---|
EGTA | 0.2 and 2 mM (0.2 M in water) | Calcium chelator [56] |
N-acetyl cysteine | 10 and 100 mM (10 M in water) | Scavenger of reactive oxygen species and protects thiol group of phosphatases from inactivation [26] |
Sodium fluoride | 1 and 10 mM (1 M in water) | Inhibitor of serine-threonine phosphatases [27] |
Sodium orthovanadate | 1 and 10 mM (1 M in water) | Inhibitor of tyrosine phosphatases [26] |
Staurosporine | 10 and 100 nM (10 μM in ethanol) | Broad range inhibitor of serine-threonine kinases |
Suramin | 0.1 and 1 mM (0.1 M in water) | Inhibits binding of growth factors to their receptors [17] |
Verapamil | 0.5 and 5 μM (0.5 mM in ethanol) | L-type calcium channel blocker [28, 29] |
PD 98059 [2-(2-amino-3-methoxyphenyl) -oxanapthalen-4-one] | 5 uM (0.5 mM in DMSO) | ERKK inhibitor [58] |
SB 203580 [4-(4-flurop henyl)-2-(4-pyridyl) 1H imidazole] | 70 nM (7 μM in DMSO) | P38 MAPK inhibitor [58] |
SB 600125 ( anthrax-[1,9-cd]-6(2H)-one] | 40 nM (4 μM in DMSO) | JNK inhibitor [59] |