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Figure 2 | BMC Plant Biology

Figure 2

From: UV-B-induced signaling events leading to enhanced-production of catharanthine in Catharanthus roseuscell suspension cultures

Figure 2

Production of ROS in C. roseus suspension cultured cells in response to UV-B irradiation. (a) A time course of UV-B induced ROS production. Six-day-old cell suspension cultures were irradiated by UV-B for different times and 2.5 μM DCFH-DA was added. The ROS production was measured after 15 min as a difference in the fluorescence intensity between the UV-B-irradiated and untreated controls. Bars represent means ± SD (n = 3). (b) Effect of various inhibitors on UV-B induced ROS production. Six-day-old cell suspension cultures were treated with 1 mM suramin (Sur), 10 mM N-acetyl cysteine (NAC), 0.5 μM verapamil (Vera), 10 nM staurosporine (St), 40 nM SB 600125, a JNK inhibitor (SB6), 70 nM SB 203580, a P38 inhibitor (SB2) and 5 μM PD 98059, an ERKK inhibitor (PD) for 10 min prior to UV-B irradiation of 5 min and 2.5 μM DCFH-DA was added to the treated cultures. The ROS produced was measured as above.

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