Table 2 Summary of genes surveyed and primer sequences employed.
From: DNA sequence diversity and the origin of cultivated safflower (Carthamus tinctoriusL.; Asteraceae)
Locus | Functional Annotation via BLAST | Primer Sequences (Forward/Reverse) |
|---|---|---|
A19 | At2g21330 | 5'-CTAGAGAACACSGARGCTAACCG |
Putative fructose bisphosphate aldolase | 5'-TGGCGAAACGRGCACCYTGTTGG | |
A25 | At2g45740 | 5'-TTGCATGSTCTTATCAGTCC |
Similar to putative peroxisomal membrane protein PEX11-1 | 5'-GAAGABCCCATCCARCAGAAGAG | |
A25a | - | Same as A25 Forward |
5'-TCTCTCTCATGACACCATGTAAA | ||
A25b | - | 5'-GCTCCACAGATCAGGCATTT |
Same as A25 Reverse | ||
A27 | At3g19900 | 5'-CTTGCAWTGAATGTCATGTGGAAG |
Unknown protein | 5'-GCTCCCCARCATTTCA | |
A39 | At2g28315 | 5'-ACTAGTTGGCATYTRATGGTAACA |
Putative glucose-6-phosphate/phosphate-tranlocator | 5'-GCCRACAAAATTGAGCTGAAGATC | |
A39a | - | Same as A39 Forward |
5'-TCATGGACCAGAAATGAYGTT | ||
A39b | - | 5'-AGCACCCTTCCYTACTGCAT |
Same as A39 Reverse | ||
B7 | At4g27700 | 5'-AGAAAAYARCTTTGTGATTCTTGATG |
Contains Rhodanese Homology Domain | 5'-GAWGARCAAGCTACTATRATCTTTG | |
B12 | At3g55800 | 5'-CAAGTGGCTGCAGCCATGGG |
Sedoheptulose-bisphosphatase precursor | 5'-ACATCRGGMACCATTCCWCCGGTGT | |
B27 | At4g33250 | 5'-AAGGCTCTTATGGCHATGCC |
Similar to eukaryotic translation initiation factor 3 subunit 11 | 5'-CGGTTYTTRGCWGCTTCATCCCARAACTG | |
B27a | - | Same as B27 Forward |
5'-TGATGCAAAATAGTTTGTTGGAA | ||
B27b | - | 5'-TGGTGMTYCTTTTCCAATTC |
Same as B27 Reverse |