Association of six YFP-myosin XI-tail fusions with mobile plant cell organelles

Table 1 Primers for GATEWAY directional cloning

Primers	Myosin XI-5 (At MYA1)	Myosin XI-17 (At XI-K)
tail	CACCCTTAGAATGCTGAAAATGGCTG	CACCGCACGAGACACAGGAGC
1/2 coil	CACCGTTGTTGTTGAAGATACAG	CACCGTATTGGTTGAGGATACTG
no coil	CACCACTCTTACCATCTCACCGAC	CACCACCAGCAGAACTATGG
1/2 tail	CACCAGACGTCGGGGAATGCCTTC	CACCCAGAGGCGAAGAACAACATCAGC
dilute	CACCGTGTTCGGGCAGATAT	CACCGTATTCACACAAATATTCTCC
reverse	TCAATCTGACCTTTCCAACAAG	TTACGATGTACTGCCTTCTTTACG
	Myosin XI-6 (At MYA2)	Myosin XI-15 (At XI-I)
tail	CACCCTTAAGATGGCTGCTAGAG	CACCCTTAAACAGGTTGCTAATG
1/2 coil	CACCGTGGTTGATCAGGAATT	CACCGCGGTACTTGAAAAGC
no coil	CACCGTGAGGACAAATCTAGGAC	CACCTCCCCAGAGAGAATAGGAC
1/2 tail	CACCGCTTCAACTTCTTTATTTGG	CACCGCTTCAGCACTTCTATGC
dilute	CACCACTTTCTCATACATTAACG	CACCCTTGTGACTCAGGTTTTCTC
reverse	CTAGTGCAAGAATACAAATGCTGG	TCAAATGATCTGCTTTGAGGTTG
	Myosin XI-8 (At XI-B)	Myosin XI-16 (At XI-J)
tail	CACCCTTAAGATGGCTGCTCG	CACCTCAAAGCAGGCTGACAG
reverse	CTAGTGCAAGAATACGAATTCTGG	TCAAAAGTAATCTTCGAAGCCC

All primers are listed 5'→3'. Forward primers contain the four bases CACC allowing directional cloning into pENTR/D TOPO vector (Invitrogen).