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Figure 3 | BMC Plant Biology

Figure 3

From: Genome-wide analysis of Aux/IAA and ARF gene families in Populus trichocarpa

Figure 3

Expression analysis of PoptrIAA3 subgroup genes using real-time RT- PCR. Fold Change (*) is represented relative to lowest value observed for the gene. Lowest value was determined by comparison of relative threshold cycle values for a specific gene across leaf, stem and root samples. Fold change was calculated by the formula 2-ΔΔCt, where ΔΔCt is the difference between ΔCt of a gene is a given tissue and the lowest value ΔCt observed for that gene in any of the three tissue types. ΔCt was estimated by the formula; (Ct of gene of interest) – (geometric mean of ΔCt of 18S RNA gene, control gene). Note that the relative fold change in expression of PoptrIAA3.2 gene in stem with respect to root (tissue with lowest PoptrIAA3.2 expression level) is 128, which has been represented on a discontinuous y-axis to capture the other lower fold change values observed.

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