CF and B. cinerea -induced expression of defense-related genes in Physcomitrella. Expression of PR-1, PAL, CHS and LOX genes was characterized by RNA-gel blot hybridization after the following treatments: moss colonies sprayed with LB (control, C), CF(SCC1), CF(SCC3193) or inoculated with B. cinerea spores (2 × 105 spores/ml). Plant samples were harvested at the indicated times (hours) after treatment. 10 μg of RNA was separated on formaldehyde-agarose gels, transferred to nylon membranes and hybridized to 32P-labeled DNA probes. Ethidium bromide staining of rRNA was used to ensure equal loading of RNA samples. Similar results were obtained from two independent experiments.