Analysis of protonemal filament changes in response to treatments with CF and B. cinerea spores. Protonemal filaments examined under transmitted light after 2 days of treatment with LB (A), CF(SCC3193) (B), CF(SCC1) (C, D), and B. cinerea spores (E). Cytoplasmic shrinkage observed with CF(SCC1) and B. cinerea spores are indicated with arrows. CF(SCC1)-treated protonemal cells showing browning of chloroplasts and loss of red chlorophyll autofluorescence after UV-excitation are indicated with arrows (F, G). A cell with collapsed cytoplasm and fewer chloroplasts is shown 4 days after treatment (indicated with an arrow) (H).