Figure 4From: Erwinia carotovora elicitors and Botrytis cinerea activate defense responses in Physcomitrella patensAnalysis of protonemal filament changes in response to treatments with CF and B. cinerea spores. Protonemal filaments examined under transmitted light after 2 days of treatment with LB (A), CF(SCC3193) (B), CF(SCC1) (C, D), and B. cinerea spores (E). Cytoplasmic shrinkage observed with CF(SCC1) and B. cinerea spores are indicated with arrows. CF(SCC1)-treated protonemal cells showing browning of chloroplasts and loss of red chlorophyll autofluorescence after UV-excitation are indicated with arrows (F, G). A cell with collapsed cytoplasm and fewer chloroplasts is shown 4 days after treatment (indicated with an arrow) (H).Back to article page