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Table 3 P. atrosepticum cultural growth conditions

From: Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum

Name

Medium

Temp (°C)

Phase (A600)

MMcap

M9 mm, cap (0.05 %), pga (0.125 %), abg (0.05 %)

15

0.4

MMg

M9 mm, glucose (0.2 %)

15

0.4

LB

LB

15

0.4

LBexp

LB

27

0.4

LBst

LB

27

2.0

MMcip

M9 mm, cip (0.5 %), pga (0.5 %), abg (0.5 %)

27

0.4

  1. M9 mm, M9 minimal medium; LB, Luria Bertani broth; cap, cabbage pectin; pga, polygalacturonic acid; abg, arabinogalactan; cip, citrus pectin. M9 minimal medium and LB medium was made according to Sambrook et al. (1989) [56]. All cultural conditions included shaking at 200 rpm.
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BMC Plant Biology

ISSN: 1471-2229

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