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Table 3 P. atrosepticum cultural growth conditions

From: Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum

Name Medium Temp (°C) Phase (A600)
MMcap M9 mm, cap (0.05 %), pga (0.125 %), abg (0.05 %) 15 0.4
MMg M9 mm, glucose (0.2 %) 15 0.4
LB LB 15 0.4
LBexp LB 27 0.4
LBst LB 27 2.0
MMcip M9 mm, cip (0.5 %), pga (0.5 %), abg (0.5 %) 27 0.4
  1. M9 mm, M9 minimal medium; LB, Luria Bertani broth; cap, cabbage pectin; pga, polygalacturonic acid; abg, arabinogalactan; cip, citrus pectin. M9 minimal medium and LB medium was made according to Sambrook et al. (1989) [56]. All cultural conditions included shaking at 200 rpm.