|
Genes
|
Average expression stability M
|
Pairwise variations V
|
|---|
|
ffh
|
0.334
|
-
|
|
recA
|
0.334
|
0.118
|
|
proC
|
0.371
|
0.166
|
|
gyrA
|
0.533
|
0.288
|
|
16S
|
0.909
|
-
|
- Ranking of the most stably expressed genes from top to bottom and determination of the optimal number of genes needed for normalisation. The average expression stability value M was obtained by calculating the M for each gene and excluding the gene with the highest M (lowest stability) for the next calculation round. The optimal number of reference genes needed for normalisation is determined by the pairwise variation (V) between the normalisation factors. The cut-off value of V was set to 0.15, below which the inclusion of another reference gene has no significant effect, as suggested by Vandesompele et al. [6].
