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Table 2 Ranking of reference genes by geNorm

From: Evaluation of reference genes for real-time RT-PCR expression studies in the plant pathogen Pectobacterium atrosepticum

Genes Average expression stability M Pairwise variations V
ffh 0.334 -
recA 0.334 0.118
proC 0.371 0.166
gyrA 0.533 0.288
16S 0.909 -
  1. Ranking of the most stably expressed genes from top to bottom and determination of the optimal number of genes needed for normalisation. The average expression stability value M was obtained by calculating the M for each gene and excluding the gene with the highest M (lowest stability) for the next calculation round. The optimal number of reference genes needed for normalisation is determined by the pairwise variation (V) between the normalisation factors. The cut-off value of V was set to 0.15, below which the inclusion of another reference gene has no significant effect, as suggested by Vandesompele et al. [6].