Immunolocalization of AtCNGC10 to the plasma membrane of diverse Arabidopsis leaf cells using transmission electron microscopy. (A) Bright field cross-section view of leaf cells examined, ep, epidermis; P, palisade parenchyma; sm, spongy mesophyll. (B) Partially plasmolyzed epidermal cell labeled with anti-AtCNGC10 antiserum and 15 nm gold secondary antibody on plasma membrane (pm, arrows). Plasma membranes of palisade parenchyma cell (C) and spongy mesophyll cell (D) labeled with anti-AtCNGC10 antiserum and 10 nm gold secondary antibody (arrows). Cell wall, cw, and cytoplasm, cy, are denoted in each panel. Size bars are in microns (μ) or nanometers (nm). Controls with secondary antisera alone gave no signal (data not shown).