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Figure 3 | BMC Plant Biology

Figure 3

From: The cyclic nucleotide gated cation channel AtCNGC10 traffics from the ER via Golgi vesicles to the plasma membrane of Arabidopsis root and leaf cells

Figure 3

Immunolocalization of AtCNGC5 and AtCNGC10 to the plasma membrane of Arabidopsis leaf protoplasts using confocal laser scanning microscopy. Wild type leaf protoplasts were challenged with primary antibodies specific to AtCNGC5 (C5), and AtCNGC10 (C10), followed by AlexaFluor488 anti-rabbit secondary antibodies. A negative control using only the secondary antibody was also performed (2°). Exposures included (g) a 505–525 nm emission filter for AlexaFluor488, (r) chlorophyll autofluorescence detected using a 650 nm emission filter, or (rg) both immunofluorescence and chlorophyll autofluorescence merged.

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BMC Plant Biology

ISSN: 1471-2229

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