Figure 2

Phenotypes of transgenic Arabidopsis plants with different tagging constructs. (A) Wild-type Arabidopsis at the rosette stage, (D) at the inflorescence stage, and (G) a close-up of a flower. (B) Line with AG-eGFP fusion construct showing an AG overexpression phenotype (pARC276). (C) Line with SEP3-eGFP fusion construct showing a SEP3 overexpression phenotype (pARC277). Rosette stage images (A-C) were taken from plants grown under the same conditions and were of the same age (bar indicates relative size). (E, H) Line with eGFP-AG fusion construct showing an ag mutant phenotype (pARC308). (F, I) Line with eGFP-SEP3 fusion construct showing a partial sep-like mutant phenotype (pARC309). (J) Siliques of lines with GFP-FUL fusion construct with either a FUL overexpression (FUL), ful mutant (ful) phenotype, or wild-type phenotype (WT) (pARC310). (K) Arabidopsis root tip and (L) open silique with an ovule of a line expressing GFP-FUL fusion construct (pARC310) observed by fluorescence microscopy. dz, dehiscence zone; v, valve; ov, ovule; n, nuclues; ca, carpel wall.