Table 1 Summary information on allele sequences for 11 candidate genes obtained from the 20 diploid heterozygous L. perenne genotypes within the LTS
Genes | Homologues | Fragment length (bp)a | Sequenced (bp)b | No. of haplotypes | Primers for gene fragments (5' to 3') |
|---|---|---|---|---|---|
EST1 | NBS-LRR | 949 | 949 | 27 | F ctcggatccaacatggagga R tgttgtcttgccaataccgc |
EST6 | NBS-LRR | 936 | 932 | 27 | F gggaaagtccaaacttgacg R cgtaagaatgggtgaaaggt |
EST7 | NBS-LRR | 1036 | 1036 | 19 | F atagccttcctttggcaatc R ctggacaacgagttacacgg R |
EST26 | NBS-LRR | 1140 | 507 + 523 | 14 | F gctgcagctggctaacaaca R ccaaatgtgccagcaactgc |
EST31 | NBS-LRR | 943 | 943 | 12 | F agcacgccatcactgttcta R ctagggcatcaaccgactgt |
EST45 | NBS-LRR | 1014 | 1056 | 23 | F gagcagccttcctccaaact R caaggccacgagaactagca |
EST13 | EDR-1 | 2200 | 480 + 479 | 10 | F aagcggaggattaagatggc R cacatattcacatgggacgc |
EST24 | MAP-1 | 1350 | 505 + 480 | 13 | F tatatccgccaacttccccg R tcaatcatcacctgcccacc |
EST28 | PKPA | 1145 | 509 + 510 | 10 | F gagcaacaagactgaccatt R caatctggtttgttcttggc |
EST39 | PKPA | 1500 | 502 + 475 | 15 | F cacatcatcggattccacaa R atacatcccaatccacctgg |
EST40 | PRP-1 | 1085 | 1085 | 9 | F agaaacaggaggcgacaagt R ggagtgatcgtccttttaca |