Figure 5

Comparison of expression levels of castor FAD2 , FAH12 and oleosin ( OLE2 ) genes in developing endosperm by RT-PCR analysis. (a, d) FAD2; (b, e) FAH12; (c, f) OLE2. PCR conditions are 94°C 30s, 55°C 30s and 72°C 1min for 15 cycles (a, b, c) or 25 cycles (d, e, f). Equal amount (3 μL) of PCR reactions (total 20 μL) were loaded for electrophoresis.