Expression of the Tgm-Express1 captured gene fragments and related host genes in the Wp -flower color isolines. (A) RNA gel blot with total RNA samples purified from flower buds, seed coats of three developmental stages and cotyledons of two developmental stages of the flower color isolines: LN89-5320-8-53 (wpmwpm), LN89-5320-6 (WpWp) and LN89-5322-2 (wpwp). Seed fresh weight of each seed coat and cotyledon sample in mg is shown at bottom. The chimeric DNA probe containing UP, CDC2, FPK, M and CS sequences was an amplification product from the seed coat RT-PCR derived wp-12 cDNA clone (Figure 3). (B) Ethidium bromide-stained gel prior to membrane transfer. The 25 S rRNA is shown to compare RNA sample loading.