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Figure 2 | BMC Plant Biology

Figure 2

From: Novel exon combinations generated by alternative splicing of gene fragments mobilized by a CACTA transposon in Glycine max

Figure 2

Variant flavanone 3-hydroxylase cDNAs from isolines containing mutant wp alleles. (A) Ethidium bromide-stained gel showing an array of cDNA bands between 5 and 1.4 kb in size that were amplified from RNAs of seed coats of the wp mutant line LN89-5322-2 through RT-PCR reactions. The (+) and (-) at top indicate reactions with and without Superscript RTII. The bright broad bands obtained from mutant RNA samples in the (+) reactions were resolved into a group of discreet bands with shorter photographic exposure of the same gel (far left lane). (B) Ethidium bromide-stained gel showing cDNAs amplified via RT-PCR with RNA from cotyledons of the LN89-5322-2 (wp) mutant line.

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