Table 2 Summary of EST-SSR searches for the Lolium perenne genotypes NV#20F1-30, NV#20F1-39, and F6, and for the combined dataset.

Total number of sequences examined:

9,177

4,394

12,173

25,744

Total size of examined sequences (bp):

3,846,707

1,751,833

2,932,559

8,531,099

Total number of identified SSRs:

353

174

1,074

1,601

Number of SSR containing sequences:

327

161

970

1,458

Number of sequences containing more than 1 SSR:

25

13

95

133

Number of SSRs present in compound formation:

15

6

75

96

Repeat types

Di-nucleotide type:

58

45

92

195

Tri-nucleotide type:

237

102

914

1,253

Tetra-nucleotide type:

54

25

47

126

Penta-nucleotide type:

4

1

13

18

Hexa-nucleotide type:

0

1

8

9

Number of ESTs per SSR:

26.0

25.3

11.3

16.1

Kb sequence per SSR:

10.9

10.1

2.7

5.3