Figure 7
Binding of the EDR2 PH-domain to lipids. GST-tagged EDR2 PH domain was affinity purified and used to probe blots spotted with various lipids. The PH-GST fusion proteins were detected with an anti-GST antibody. PHD-1: wild-type PH-domain of EDR2; PHD-2: mutated PH-domain of EDR2 (F93S); GST: glutathione S-transferase negative control. Compounds spotted on the membrane: 1, lysophosphatidic acid; 2, lysophosphatidylcholine; 3, phosphatidyl inositol; 4, phosphatidylinositol 3-phosphate; 5, phosphatidylinositol 4-phosphate; 6, phosphatidylinositol-5-phosphate; 7, phosphatidyl ethanolamine; 8, phosphatidyl choline; 9, sphingosine-1-phosphate; 10, phosphadidylinositol-3,4-phosphate; 11, phosphadidylinositol-3,5-phosphate; 12, phosphatidylinositol-4,5-phosphate; 13, phosphadidylinositol-3,4,5-phosphate; 14, phosphatidic acid; 15, phosphatidyl serine; 16, blank