Transcriptional activation and localization of expression of Brassica juncea putative metal transport protein BjMTP1

Table 1 Different abiotic stress responses of 983 bp promoter element of BjMTP1

Different stress treatments	GUS activity (nmoles of 4 -methyl umbelliferrone min^-1 mg^-1 protein)
Cold shock control	81.01 ± 3.89
Cold shock	87.7 ± 4.61
Heat shock control	84.55 ± 3.13
Heat shock	85.21 ± 6.46
NaCl stress control	99.1± 5.71
100 mM NaCl stress	98.38 ± 8.92
H₂O₂ control	91.46± 3.07
50 μM H₂O₂ stress	96.16± 0.68

Five-day-old dark grown seedlings stably transformed with p(1.0)BjMTP1::GUSPlus were incubated at 4°C for 4 h (cold shock), 37° C for 2 h (heat shock),100 mM NaCl (24 h) and 50 μM H₂O₂ (24 h) and GUS activity measured. Data represents the average (± SD) of three independent replicate samples.

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