Transcriptional analysis of PME1 using RT-PCR. RNA was extracted from hypocotyls after 2 d growth on control/induction medium (arrows in Figure 3) and reverse transcribed. PME1 expression was detected using gene-specific primers to amplify a 932 bp product. Actin isoform 2-specific primers were used as controls. Lanes denote treatment, (-) no ethanol, and (+) 0.1% ethanol.